------------- EXCEPTION: Bio::Root::Exception -------------
MSG: Could not open Perm.txt for reading: No such file or directory
STACK: Error::throw
STACK: Bio::Root::Root::throw F:/Perl/site/lib/Bio/Root/Root.pm:342
STACK: Bio::Root::IO::_initialize_io F:/Perl/site/lib/Bio/Root/IO.pm:260
STACK: Bio::SeqIO::_initialize F:/Perl/site/lib/Bio/SeqIO.pm:437
STACK: Bio::SeqIO::raw::_initialize F:/Perl/site/lib/Bio\SeqIO\raw.pm:94
STACK: Bio::SeqIO::new F:/Perl/site/lib/Bio/SeqIO.pm:355
STACK: Bio::SeqIO::new F:/Perl/site/lib/Bio/SeqIO.pm:368
STACK: -:31
-----------------------------------------------------------